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PFGE is based on the electrophoretic separation of extremely large DNA, raising the upper size limit from 50 kb (standard agarose separation) to well over 10 Mb. ĪBSTRACT: To circumvent the limitations of cultivation-based studies of complex microbial communities, molecular fingerprinting techniques such as pulsed field gel electrophoresis (PFGE) and denaturing gradient gel electrophoresis (DGGE) have been used to examine their richness, diversity, and dynamics.

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Suttle, Manual of Aquatic Viral Ecology. Fingerprinting aquatic virus communities, p. The approaches have great potential for elucidating trends in virus production rates as well as for making generalized estimates of the quantitative effects of viruses on marine microbial communities.įull Citation: Sandaa, R.-A., S. Several modifications of the method are presented and compared. This reoccurrence can be quantified and used to assess production and cell lysis rates.

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This allows for easy enumeration of viruses that originate from lysis of already infected cells due to the decreased abundance of free virus particles. ĪBSTRACT: The reduction approach to assess virus production and the prokaryotic mortality by viral lysis stops new infection by reducing total virus abundance (and thus virus-host contacts). Determining rates of virus production in aquatic systems by the virus reduction approach, p. Waco, TX:American Society of Limnology and Oceanography. For the entire book, a suggested citation is as follows. Citations of each chapter should follow the form recommended in its acknowledgments. The content of this e-book was selected in consultation with the Scientific Committee for Oceanographic Research's working group on marine viruses, and its publication has been supported by the Gordon and Betty Moore Foundation.Ĭhapters in the MAVE e-Book are freely available for download. Topics range from the enumeration of viruses to molecular techniques designed to dissect and query individual virus populations as well as communities of viruses. It contains 19 chapters reflecting state-of-the-art opinions on approaches to studying viruses in aquatic systems. Articles about ASLO and Science Menu ToggleĪSLO's first e-Book publication is the Manual of Aquatic Viral Ecology (MAVE), edited by Steven Wilhelm, Markus Weinbauer and Curtis Suttle.Ecological Dissertations in the Aquatic Sciences Menu Toggle.2023 Global Outreach Initiative Proposal Submission.Call for Applications: Raelyn Cole Editorial Fellowship.Diverse Voices in the Aquatic Sciences – Virtual Issue.

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